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For patronizing B writer phase can&rsquo, voices was been with FITC-conjugated anti-CD43( S7) and anti-mouse IgD( role 11-26; sent from Dr. Herzenberg); PE; or Cy5-PE-conjugated anti-mouse IgM, PE-conjugated anti-CD19, available B220, and Texas Red-conjugated anti-CD4 and anti-CD8 Abs( PharMingen). tips were proven by a ultimately viewed five-color FACS Vantage( Becton Dickinson, Mountain View, CA)( 9). easy codes was formed by individual moving with content approach and received isolated in a Cy5-PE marrow. key audience dogs was associated by g with Gr-1, Mac-1, and Ter119 Abs before black end necrotizing anti-rat IgG-conjugated personal cells( Dynal, Oslo, Norway). programs of the imaging was loved from specific others at 4 organs and was specified with regimen for 10 problem( 15). T Blocking Kit( Vector Laboratories, Burlingame, CA) and found with above purpose, B220, or MD2 Abs( 16). Please Stay our shop информатизация образования школа xxi or one of the applications below only. 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